- Easy-to-use and cost-effective method for highly efficient viral concentration
- Concentrate all lentiviral particles 10 to 100 times with PEG-it
- Freeze pseudo viral particles without loss of titer
- Non-toxic reagents safe to use with all tested target cell lines, including embryonic stem cells
- No special equipment required – avoid the expense of an ultracentrifuge
- No accumulation of cellular debris as occurs with ultracentrifugation; avoid toxic and immunogenic reactions by using PEG-it instead
- Ideal for concentrating large volumes of viruses – avoid the spin-limited capacity of ultracentrifugation
- Improve cell transduction and prepare ultra-high titer preparations for in vivo infections
- Sterile and ready to use in cell culture.
- Couple precipitated virus with TransDux for high infectivity
- Fully compatible with the System Bioscience LentiMag magnetotransduction kit
Concentrate viral particles 100 times
The PEG-it Virus Precipitation Solution provides a simple and highly efficient means of concentrating lentiviral particles, such as those produced with the pPACK Lentivector packaging system from System Biosciences. The PEG-it Virus Precipitation Solution is a polyethylene glycol formulation optimized for the precipitation of all lentiviral particles. The solution is mixed with virus-containing cell culture supernatant, incubated at 4 ° C overnight, and centrifuged at 1500 xg to pellet the precipitated viral particles. As a result, the concentrations of viral particles increase 10 to 100 times.
PEG-it protects the virus from multiple freeze/thaw cycles
The PEG-it virus not only concentrates your lentil preparations but also acts as a cryopreservative agent. PEG-concentrated lentivirus lasts longer in the freezer and also survives fairly well in aliquot freeze/thaw cycles. To test this, 50,000 HepG2 or HT1080 cells were seeded in 500 microliters of DMEM medium containing 10% FBS in 24-well plate formats. The next day, the medium was replaced with a fresh medium containing 1x TransDux virus transduction reagent.
Pre-prepared frozen aliquots of virus packaged with an MSCV-GFP-T2A-RFP expression cassette were thawed on ice and 2 ul of the virus was added to each well in triplicate. Aliquots were immediately frozen on dry ice and thawed again on normal ice and 2ul of the virus was again added to three other wells. This process was repeated three more times. Seventy-two hours after transduction, cells were imaged for green fluorescence and documented. The images below show how well PEG-it protected the virus from multiple freeze/thaw cycles and produced high levels of transgene expression of the GFP and RFP virus.