AlbuTrial Kit – AlbuVoid and AlbuSorb
The AlbuTrial kit includes 1 g of AlbuSorb and 5 AlbuVoid preparations with their respective buffers. Matrix
facilitating the efficient removal of albumin from serum. Proteomic analysis of serum and search for identification of serum proteins as disease markers have often been hampered by the prevalence of proteins such as albumin. The unusually high abundance of serum albumin suppresses the signals of low abundance proteins, which interferes with the resolution and sensitivity of many protein profiling techniques.
In the course of evaluating various available methods and commercial kits, have been able to refine albumin depletion protocols and establish a modified albumin clearance method using a ligand-based protein fractionation system. absorb and AlbuVoid matrices were tested in humans, sheep, cattle, mice, goats, rats, and calves from serum and plasma using a simple protocol.
The procedure is quite simple, just add, centrifuge, and/or filter, and recover the albumin-depleted serum. AlbuSorb facilitates the binding of albumin (> 90%) thus eliminating the rest of the proteins in the continuous flow. AlbuVoid, on the other hand, removes most of the albumin (> 90%) in the flow and wash fractions. In the elution step, albumin-depleted serum proteins can be recovered.
Albumin reduction plus low abundance serum/plasma protein enrichment
- Continuous flow albumin voids> 95%, with binding / washing / elution protocol of <30 minutes
- Low abundance enrichment equivalent to or better than hexapeptides or antibodies
- Disposable, cost-effective, no column regeneration or cross-contamination
- Mild elution maintains the tertiary structure and simple transfer to secondary analysis
- The eluted fractions retain their enzymatic and biological activity
- Removes albumin from many species, including humans, mice, sheep, cattle, goats, rats, and calf from serum and plasma.
AlbuVoid, a silica-based protein enrichment matrix, removes albumin from serum and plasma samples while concentrating low abundance and/or low molecular weight proteins. The AlbuVoid protocol uses soft buffers; The conditions of the protocol are so mild that the activity of the native enzyme is retained in elution fractions. AlbuVoid significantly improves protein resolution below 50 kD, a limitation of alternative enrichment protocols.
AlbuVoid is derived from a silica-based library of individual mixed-mode ligand combinations (ionic, hydrophobic, aromatic, polymer). The library was designed to facilitate the weak binding of proteins, allowing rapid elution of the matrix without any prior knowledge of the variety of proteins contained in the initial sample. In contrast to traditional chromatographic methods, our weak binding approach is more selective, presumably due to a lower degree of non-specific protein-protein interactions at the surface interface.
In the case of AlbuVoid, a unique mixed-mode ligand the architecture was empirically selected from the library. Due to its specific binding properties, AlbuVoid depletes high abundance serum proteins such as albumin and immunoglobulins while improving the resolution of less abundant serum proteins.
Serum or plasma albumin depletion
- AlbuSorb binds at approximately 30 mg albumin/ml and serum proteins flow through.
- Affinity type equivalence, practically no cross-reactivity with other proteins
- Disposable, cost-effective, and without cross-contamination
- New inexpensive surface technology, not based on affinity chromatography
- The mild condition maintains the tertiary structure and the simple transfer to the secondary analysis
- The reduced flow of albumin through the fractions retains its enzymatic and biological activity.
- Removes> 90% of albumin from many species, including human, sheep, bovine, mouse, goat, rat,
and veal from serum and plasma.
Polyelectrolytes are polymers with repeating units of stationary charges. AlbuSorb comes from a class of solid phase or surface-based elastomeric polyelectrolyte surfaces that bind to proteins through empirically derived chemistry combining elements of polymer composition, crosslinking architecture, and load properties. Unlike immunoaffinity, the surfaces used are disposable eliminating cycle-to-cycle variation and cross-contamination. AlbuSorb is supplied in powder form. Simply weigh, centrifuge, and/or filter and recover the albumin-depleted serum in the supernatant.